Research Article
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Determination of antioxidant and antimicrobial activities of bilberry (Vaccinium myrtillus L.) extrackts in different solvents

Year 2017, Volume: 18 Issue: 1, 21 - 27, 01.04.2017
https://doi.org/10.17474/artvinofd.271088

Abstract



In this study, both fresh and dried fruit, leaves extracts of bilberies (V. myrtillus L.) growed in Giresun have been investigated in four different solvents (ethanol, acetone, water and diethyl ether). Several methods have been utilized to evaluate the antioxidant capacity of plant materials. These methods include FRAP (ferric ion reducing antioxidant power), DPPH● (free radical) and analysis of total polyphenol. The reducing powers of extract have been acetone > ethanol > water > diethyl ether respectively. According to free radical scavenging activity results, the extracts obtained dried samples have had better DPPH radical scavenging results than fresh ones and the dried leaves extracts in ethanol have showed the highest activity. According to analysis of total phenol, the phenolic contents in extracts have been determined as gallic acid equivalent ranging from 0.121±0.00 to 19.484±1.85 mg/g and the dried bilberry leaves extracts in water have showed the highest activity. In addition, antimicrobial activities of plant extracts have been determined by the agar disk diffusion method. The extracts with ethanol and acetone among all bilberry extract have been showed a good degree of activity against most of tested microorganisms.


References

  • Abay E (2006) Bazı Bitkilerin Ekstraktlarının Antimikrobiyal Etkilerinin Disk Difüzyon Yöntemiyle Araştırılması, Yüksek Lisans Tezi, Kars.
  • Akagün G (2009) Alabaş (Brassica oleracea var. Gongylodes) Bitkisinin Antioksidan Aktivitesinin İncelenmesi, Yüksek Lisans Tezi, Edirne.
  • Akkuş İ (1995) Serbest radikaller ve fizyopatolojik etkileri, Mimoza Yayınları, Konya.
  • Almaraz N, Campos M, Avila J.A, Naranjo N, Herrera J, and Gonzalez L.S (2007) Antioxidant activity of polyphenolic extract of monofloral honeybee collected pollen from mesquite (Prosopis juliflora, Leguminosae). J Food Compos Anal. 20(2): 119–24.
  • Atalay M, Gordillo G, Roy S, Rovin B, Bagchi D, Bagchi M, and Sen C.K (2003) Anti-Antigionic Property of Edible Berry in a Model of Hemangioma. FEBS Letters 544: 252-257.
  • Bauer A.W, Kırbyrby W.M.M, Sherrıs J.C, and Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am. J. clin. Pathol 45: 493– 496.
  • Benzie Iff, Strain Jj (1996) The ferric reducing ability of plasmav (FRAP) assay a measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry 239: 70-76.
  • Beretta G, Granata P, Ferrero M, Orioli M, Facino R.M (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta 533(2): 185-191.
  • Bradshaw L.J (1992) Laboratory of microbiology, 4th edn, Saunders College Publishing, USA, pp 435.
  • Bravo L (1998) Polyphenol chemistry, dietary sources, metabolism and nutritional significance, Nutr. Rev. 56: 317-333.
  • Burdulis D, Sarkınas A, Jasutiene I, Stackeviciene E, Nicolajevas L, Janulis V (2009) Comparative Study Of Anthocyanın Composition, Antimicrobial And Antioxidant Activity In Bilberry (Vaccinium Myrtillus L.) And Blueberry (Vaccinium Corymbosum L.) Fruits. Acta Poloniae Pharmaceutican Drug Research 66(4): 399-408.
  • Collins C.H, Lyne P.M, and Grange J.M (1989) Microbiological methods. 6th edn, Butterworths, London, pp 410.
  • Çelik H (2006) Karadeniz Bölgesindeki asitli topraklar için mükemmel bir meyve, likapa (yaban mersini). Çiftçi Dünyası Of Ziraat Odası Yayın Organı 2(2): 2-5.
  • Evers D.L, Chao C.F, Wang X, Zhang Z.G, Huong S.M, and Huang E.S (2005). Human cytomegalovirus-inhibitory flavonoids: studies on antiviral activity and mechanism of action. Antiviral Res. 68(3): 124–34.
  • Faria A, Oliveira J, Neves P, Gameiro P, Santos-Buelga C, Freitas V (2005) Antioxidant properties of prepared blueberry (Vaccinium myrtillus) extracts. Journal of Agricultural and Food Chemistry 53(17): 6896-6902.
  • Frankel E.N, Meyer A.S (2000) The problems of using one‐dimensional methods to evaluate multifunctional food and biological antioxidants. Journal of the Science of Food and Agriculture 80(13): 1925-1941.
  • Harris G.K, Qian Y, Leonard S.S, Sbarra D.C, and Shi X.L (2006). Luteolin and chrysin differentially inhibit cyclooxygenase-2 expression and scavenge reactive oxygen species but similarly inhibit prostaglandin-E2 formation in RAW 264.7 cells. J Nutr. 136(6): 1517– 1521.
  • Huang D, Ou B, Prior R.L (2005) The chemistry behind antioxidant capacity assays. Journal of agricultural and food chemistry 53(6): 1841-1856.
  • Hussain T, Arshad M, Khan S, Satar H, Qureshi MS (2011) In Vitro Screening of Methanol Plant Extracts for Their Antibacterial Activity. Pak. J. Bot. 43: 531-538.
  • Janovska D. Kubikova K, Kokoska L (2003) Screening for Antimicrobial Activity of Some Medicinal Plants Species of Traditional Chinese Medicine. Czech J. Food Sci. 21: 107-110.
  • Kırca A, Bilişli A, Demirel N.N, Turhan H. ve Arslan E (2007) Çanakkale florasındaki bazı tıbbi ve aromatik bitkilerin antioksidan ve antimikrobiyal aktiviteleri. TÜBİTAK Proje No: 104 0 292. Çanakkale.
  • Kil H.Y, Seong E.S, Ghimire B.K, Chung M, Kwon S.S, Goh E.J, Heo K, Kim M.J, Lim J.D, Lee D, Yu C.Y (2009) Antioxidant and antimicrobial activities of crude Sorghum ekstract, Food Chemistry 115: 1234-1239.
  • NCCLS National Committee for Clinical Laboratory Standarts. (2000). Performance standarts for antimicrobial disc susceptility tests; 7 ed. Approved Standart M2-A7 NCCLS, Pennsylvania.
  • Silva S, Costa E.M, Pereira M.F, Costa M.R, Pintado M.E (2013) Evaluation of the antimicrobial activity of aqueous extracts from dry Vaccinium corymbosum extracts upon food microorganism, Food Control 34: 645-650.
  • Singh B, Dutt N, Kumar D, Singh S, Mahajan R (2011) Taxonomy, Ethnobotany and Antimicrobial Activity of Cro-ton bonplandianum, Euphorbia hirta and Phyllanthus fraternus. J. Adv. Develop. Res. 2: 21-29.
  • Singleton V, Rossi J.A (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3): 144-158.
  • Toroglu, S (2007) In vitro antimicrobial activity and antagonistic effect of essential oils from plant. species., J. environ. Biol. 28: 551-559.
  • Tsai P.J, McIntosh J, Pearce P, Camden B, Jordan B.R (2002) Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.) extract. Food Research International, 35(4): 351-356.
  • Ünal E (2006) Türkiye florasında doğal olarak yetişen bazı bitki türlerinin antimikrobial ve antioksidan aktivitelerinin incelenmesi, Yüksek Lisans Tezi, Kırıkkale.
  • Ünal E. L, Mavi A, Kara A.A, Cakir A, Şengul M, Yildirim A (2008) Antimicrobial and antioxidant activities of some plants used as remedies inTurkish medicine, Pharmaceutical Biology 46: 207-224.
  • Wollgast J, and Anklam E (2000) Review on Polphemols in Theobroma Cacao, Changes in Composition During the Manufacture of Choconate and Methodology for Identication and Quantification. Food Res Int. 33: 423-447.
  • Wu X, Cao G, and Prior R.L (2002) Absorption and Metabolism of Anthocyanins in Elderly Women after Consumption of Elderberry or Blueberry. The Journal of Nutrition, ProQuest Agriculture Journals 132(7): 1865-1871.

Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi

Year 2017, Volume: 18 Issue: 1, 21 - 27, 01.04.2017
https://doi.org/10.17474/artvinofd.271088

Abstract

Bu çalışmada Giresun ilinde yetişen yaban mersininin (Vaccinium myrtillus L.) meyve ve yaprağı, hem yaş hem de kurutulmuş halinin dört farklı çözücüdeki ekstraktları (etanol, aseton, su ve dietil eter) ayrı ayrı incelenmiştir. Bitkisel ürünlerin antioksidan kapasitelerini değerlendirmek için çeşitli yöntemler kullanılmıştır. Bu yöntemler, FRAP (Fe3+ İndirgeme Antioksidan Gücü), DPPH● giderme ve toplam polifenol tayinini içermektedir. FRAP tayini yönünden, ekstraktların indirgeme güçlerinin aseton > etanol > su > dietil eter şeklinde sıralandığı görülmüştür. Serbest radikal giderme aktivitesi antioksidan sonuçlarına göre, kurutulmuş halde alınan örneklerin ekstraktları, yaş haline göre daha iyi düzeyde DPPH radikali giderme aktivitesi sergilemiştir ve en yüksek aktiviteyi çözücüsü etanol olan kuru yaprak ekstraktının gösterdiği bulunmuştur. Toplam fenolik madde miktarı tayinine göre ise, ekstraktlarda var olan fenolik madde içeriklerinin gallik asit eşdeğeri cinsinden 0.121±0.00-19.484±1.85 mg/g aralığında değiştiği ve en yüksek aktiviteyi yaban mersininin çözücüsü su olan kuru yaprak ekstraktının gösterdiği belirlenmiştir. Aynı zamanda bitki ekstrelerindeki antimikrobiyal aktivite, Agar disk difüzyon yöntemi kullanılarak tespit edilmiştir. Yaban mersini bitkisi ile hazırlanan ekstraktlar arasında, çözücüsü etanol ve aseton olan ekstrakların test mikroorganizmalarının çoğuna karşı iyi derecede aktivite gösterdiği bulunmuştur.


References

  • Abay E (2006) Bazı Bitkilerin Ekstraktlarının Antimikrobiyal Etkilerinin Disk Difüzyon Yöntemiyle Araştırılması, Yüksek Lisans Tezi, Kars.
  • Akagün G (2009) Alabaş (Brassica oleracea var. Gongylodes) Bitkisinin Antioksidan Aktivitesinin İncelenmesi, Yüksek Lisans Tezi, Edirne.
  • Akkuş İ (1995) Serbest radikaller ve fizyopatolojik etkileri, Mimoza Yayınları, Konya.
  • Almaraz N, Campos M, Avila J.A, Naranjo N, Herrera J, and Gonzalez L.S (2007) Antioxidant activity of polyphenolic extract of monofloral honeybee collected pollen from mesquite (Prosopis juliflora, Leguminosae). J Food Compos Anal. 20(2): 119–24.
  • Atalay M, Gordillo G, Roy S, Rovin B, Bagchi D, Bagchi M, and Sen C.K (2003) Anti-Antigionic Property of Edible Berry in a Model of Hemangioma. FEBS Letters 544: 252-257.
  • Bauer A.W, Kırbyrby W.M.M, Sherrıs J.C, and Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am. J. clin. Pathol 45: 493– 496.
  • Benzie Iff, Strain Jj (1996) The ferric reducing ability of plasmav (FRAP) assay a measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry 239: 70-76.
  • Beretta G, Granata P, Ferrero M, Orioli M, Facino R.M (2005) Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta 533(2): 185-191.
  • Bradshaw L.J (1992) Laboratory of microbiology, 4th edn, Saunders College Publishing, USA, pp 435.
  • Bravo L (1998) Polyphenol chemistry, dietary sources, metabolism and nutritional significance, Nutr. Rev. 56: 317-333.
  • Burdulis D, Sarkınas A, Jasutiene I, Stackeviciene E, Nicolajevas L, Janulis V (2009) Comparative Study Of Anthocyanın Composition, Antimicrobial And Antioxidant Activity In Bilberry (Vaccinium Myrtillus L.) And Blueberry (Vaccinium Corymbosum L.) Fruits. Acta Poloniae Pharmaceutican Drug Research 66(4): 399-408.
  • Collins C.H, Lyne P.M, and Grange J.M (1989) Microbiological methods. 6th edn, Butterworths, London, pp 410.
  • Çelik H (2006) Karadeniz Bölgesindeki asitli topraklar için mükemmel bir meyve, likapa (yaban mersini). Çiftçi Dünyası Of Ziraat Odası Yayın Organı 2(2): 2-5.
  • Evers D.L, Chao C.F, Wang X, Zhang Z.G, Huong S.M, and Huang E.S (2005). Human cytomegalovirus-inhibitory flavonoids: studies on antiviral activity and mechanism of action. Antiviral Res. 68(3): 124–34.
  • Faria A, Oliveira J, Neves P, Gameiro P, Santos-Buelga C, Freitas V (2005) Antioxidant properties of prepared blueberry (Vaccinium myrtillus) extracts. Journal of Agricultural and Food Chemistry 53(17): 6896-6902.
  • Frankel E.N, Meyer A.S (2000) The problems of using one‐dimensional methods to evaluate multifunctional food and biological antioxidants. Journal of the Science of Food and Agriculture 80(13): 1925-1941.
  • Harris G.K, Qian Y, Leonard S.S, Sbarra D.C, and Shi X.L (2006). Luteolin and chrysin differentially inhibit cyclooxygenase-2 expression and scavenge reactive oxygen species but similarly inhibit prostaglandin-E2 formation in RAW 264.7 cells. J Nutr. 136(6): 1517– 1521.
  • Huang D, Ou B, Prior R.L (2005) The chemistry behind antioxidant capacity assays. Journal of agricultural and food chemistry 53(6): 1841-1856.
  • Hussain T, Arshad M, Khan S, Satar H, Qureshi MS (2011) In Vitro Screening of Methanol Plant Extracts for Their Antibacterial Activity. Pak. J. Bot. 43: 531-538.
  • Janovska D. Kubikova K, Kokoska L (2003) Screening for Antimicrobial Activity of Some Medicinal Plants Species of Traditional Chinese Medicine. Czech J. Food Sci. 21: 107-110.
  • Kırca A, Bilişli A, Demirel N.N, Turhan H. ve Arslan E (2007) Çanakkale florasındaki bazı tıbbi ve aromatik bitkilerin antioksidan ve antimikrobiyal aktiviteleri. TÜBİTAK Proje No: 104 0 292. Çanakkale.
  • Kil H.Y, Seong E.S, Ghimire B.K, Chung M, Kwon S.S, Goh E.J, Heo K, Kim M.J, Lim J.D, Lee D, Yu C.Y (2009) Antioxidant and antimicrobial activities of crude Sorghum ekstract, Food Chemistry 115: 1234-1239.
  • NCCLS National Committee for Clinical Laboratory Standarts. (2000). Performance standarts for antimicrobial disc susceptility tests; 7 ed. Approved Standart M2-A7 NCCLS, Pennsylvania.
  • Silva S, Costa E.M, Pereira M.F, Costa M.R, Pintado M.E (2013) Evaluation of the antimicrobial activity of aqueous extracts from dry Vaccinium corymbosum extracts upon food microorganism, Food Control 34: 645-650.
  • Singh B, Dutt N, Kumar D, Singh S, Mahajan R (2011) Taxonomy, Ethnobotany and Antimicrobial Activity of Cro-ton bonplandianum, Euphorbia hirta and Phyllanthus fraternus. J. Adv. Develop. Res. 2: 21-29.
  • Singleton V, Rossi J.A (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3): 144-158.
  • Toroglu, S (2007) In vitro antimicrobial activity and antagonistic effect of essential oils from plant. species., J. environ. Biol. 28: 551-559.
  • Tsai P.J, McIntosh J, Pearce P, Camden B, Jordan B.R (2002) Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.) extract. Food Research International, 35(4): 351-356.
  • Ünal E (2006) Türkiye florasında doğal olarak yetişen bazı bitki türlerinin antimikrobial ve antioksidan aktivitelerinin incelenmesi, Yüksek Lisans Tezi, Kırıkkale.
  • Ünal E. L, Mavi A, Kara A.A, Cakir A, Şengul M, Yildirim A (2008) Antimicrobial and antioxidant activities of some plants used as remedies inTurkish medicine, Pharmaceutical Biology 46: 207-224.
  • Wollgast J, and Anklam E (2000) Review on Polphemols in Theobroma Cacao, Changes in Composition During the Manufacture of Choconate and Methodology for Identication and Quantification. Food Res Int. 33: 423-447.
  • Wu X, Cao G, and Prior R.L (2002) Absorption and Metabolism of Anthocyanins in Elderly Women after Consumption of Elderberry or Blueberry. The Journal of Nutrition, ProQuest Agriculture Journals 132(7): 1865-1871.
There are 32 citations in total.

Details

Journal Section Research Article
Authors

Şule Ceylan

Özlem Saral

Mehmet Özcan

Burhan Harşıt

Publication Date April 1, 2017
Acceptance Date November 30, 2016
Published in Issue Year 2017Volume: 18 Issue: 1

Cite

APA Ceylan, Ş., Saral, Ö., Özcan, M., Harşıt, B. (2017). Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 18(1), 21-27. https://doi.org/10.17474/artvinofd.271088
AMA Ceylan Ş, Saral Ö, Özcan M, Harşıt B. Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. ACUJFF. April 2017;18(1):21-27. doi:10.17474/artvinofd.271088
Chicago Ceylan, Şule, Özlem Saral, Mehmet Özcan, and Burhan Harşıt. “Yaban Mersininin (Vaccinium Myrtillus L.) Farklı çözücü ekstraktlarındaki Antioksidan Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 18, no. 1 (April 2017): 21-27. https://doi.org/10.17474/artvinofd.271088.
EndNote Ceylan Ş, Saral Ö, Özcan M, Harşıt B (April 1, 2017) Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 18 1 21–27.
IEEE Ş. Ceylan, Ö. Saral, M. Özcan, and B. Harşıt, “Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi”, ACUJFF, vol. 18, no. 1, pp. 21–27, 2017, doi: 10.17474/artvinofd.271088.
ISNAD Ceylan, Şule et al. “Yaban Mersininin (Vaccinium Myrtillus L.) Farklı çözücü ekstraktlarındaki Antioksidan Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 18/1 (April 2017), 21-27. https://doi.org/10.17474/artvinofd.271088.
JAMA Ceylan Ş, Saral Ö, Özcan M, Harşıt B. Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. ACUJFF. 2017;18:21–27.
MLA Ceylan, Şule et al. “Yaban Mersininin (Vaccinium Myrtillus L.) Farklı çözücü ekstraktlarındaki Antioksidan Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, vol. 18, no. 1, 2017, pp. 21-27, doi:10.17474/artvinofd.271088.
Vancouver Ceylan Ş, Saral Ö, Özcan M, Harşıt B. Yaban mersininin (Vaccinium myrtillus L.) farklı çözücü ekstraktlarındaki antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi. ACUJFF. 2017;18(1):21-7.
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