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Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters

Year 2019, Volume: 48 Issue: 1, 1 - 7, 15.02.2020

Abstract

Sclerotium rolfsii Sacc. is a polifag soil-borne fungal pathogen. This research aims to find out the mycelial compatible groups (MCG) of S. rolfsii, the differences in pathogenicity and morphological characters. All isolates collected from diseased green beans in Çanakkale during September, 2015. Twenty seven isolates in total were paired with each other in potato dextrose agar (PDA) to determine mycelial compatible groups (MCG). Pairings were scored as mycelial compatible if sclerotia formed in the interaction zone, however pairings were scored as mycelial incompatible if a barrier occurred. Three MCGs were determined. There were statistical differences in means of mycelial growth, the number and diameter of sclerotia produced. The correlation analysis exhibited that there was a negative correlation between sclerotium number-mycelial growth and sclerotium number and sclerotium diameter; however there have been positive correlation between mycelial growth and sclerotium diameters. All isolates were found to be highly virulent on tested bean seedlings. This research shows that there is phenotypic variation among isolates of S. rolfsii.

References

  • Aycock, R. 1966. Stem Rot and Other Diseases Caused by Sclerotium rolfsii or The Status of Rolf’s Fungus After 70 Years. North Carolina State University Agricultural Experiment Station. Tech. Bull. 174.
  • Biçici, M., Çınar, Ö. And Erkiliç, A. 1994. The control of stem rot caused by Sclerotium rolfsii Sacc on peanut by cultural, chemical, physical and biological methods. Tr J. Agric. For. 18: 423-435.
  • Erkılıç A., Güven, B. and Akgün, S.D. 2006. Effects of Some Plant Activators and Plant Materials on Stem Rot Disease of Peanut and Pepper Caused by Sclerotium rolfsii. The journal of Turkish Phytopathol, 34; 15-28.
  • Franke M., Brenneman, T., Stevenson, K. and Padgett. G. 1998. Sensitivity of İsolates of Sclerotium rolfsii from Peanut in Georgia to Selected Fungicides. Plant Dis. 82: 578-583.
  • Harlton, C.E., Levesque, C.A. and Punja, Z.K. 1995. Genetic Diversity in Sclerotium (Athelia) rolfsii and Related Species. Phytopathology. 85: 1269-1281.
  • Kohn, L.M., Stasoviski, E,. Carbone, I., Royers, J. and Anderson, J.B. 1991. Mycelial Incompatibility and Molecular Markers Identify Genetic Variability in Field Populations of Sclerotinia sclerotiorum. Phytopathology. 81: 480-485.
  • Kokub D., Azam, F., Hassan, A., Ansar, M., Asad, M.J. and Khanum, A. 2007. Comparative Growth. Morphological and Molecular Characterization of Indigenous Sclerotium rolfsii Strains Isolated from Different Locations of Pakistan. Pak. J. Bot.. 39(5): 1849-1866.
  • Kown, J.H. and Park, C.S. 2002. Stem Rot of Tomato Caused by Sclerotium rolfsii in Korea. Mycobiology. 30(4): 244-246.
  • Leslie, J.F. 1993. Fungal Vegetative Compatibility. Annu Rev Phytopathol. 31: 127-150.
  • Punja, Z.K. and Grogan, R. G. 1983. Hyphal Interactions and Antagonism among Field Isolates and Single-Basidiospore Strains of Athelia (Sclerotium) rolfsii. Phytopathology. 73: 1279-1284.
  • Punja, Z.K. 1985. The Biology. Ecology. and Control of Sclerotium rolfsii. Annu Rev of Phytopathol. 23: 97-127.
  • Punja, Z. K. and J. E. Rahe. 1992. Sclerotium. In: Singleton. LL. Mihail JD. Rush CM. eds. Methods for research on soilborne phytopathogenic fungi. St. Paul: APS Press. 166–170 pp.
  • Punja, Z.K. and Damiani, A. 1996. Comparative Growth. Morphology. and Physiology of three Sclerotium species. Mycologia. 88: 694-706.
  • Remesal, E. R., Jordán-Ramírez, R.M., Jiménez-Díaz, J. and NavasCortés, A. 2012. Mycelial Compatibility Groups and Pathogenic Diversity in Sclerotium rolfsii Populations From Sugar Beet Crops in Mediterranean-Type Climate Regions. Plant Pathol. 61: 739-753.
  • Saupe, S.J., 2000. Molecular Genetics of Heterokaryon Incompatibility in Filamentous Ascomycetes. Microbiol Mol Biol Rev. 64: 489-502.
  • Xie, C., Huang, C.H., and Vallad, G. E. 2014. Mycelial compatibility and pathogenic diversity among Sclerotium rolfsii isolates in the southern United States. Plant Dis. 98:1685-1694.
  • Yaqub, F. and Shahzad, S. 2005. Pathogencity of Sclerotium rolfsii on Different Crops and Effect of inoculum Density on Colonization Of Mungbean and Sunflower Roots. Pak. J. Bot.. 37(1): 175-180.

Sclerotium rolfsii İzolatlarının Miselyal Uyum Gruplarının Saptanması ve Morfolojik ve Patojenik Olarak Karşılaştırılması

Year 2019, Volume: 48 Issue: 1, 1 - 7, 15.02.2020

Abstract

Sclerotium rolfsii Sacc. polifag toprak kökenli fungal etmendir. Bu çalışmanın amacı S. rolfsii izolatları içinde miselyal uyum gruplarının (MUG), patojenisitelerinin ve morfolojik farklılıklarının saptanmasıdır. Tüm izolatlar Çanakkale’de fasulye üretim alanlarında, Eylül, 2015 tarihinde toplanmıştır. İzolatlar arasında MUG gruplarının saptanabilmesi için toplam 27 izolat patates dekstroz agar (PDA) ortamında eşleştirilmiştir. PDA üzerinde interaksiyon noktasında birleşme olur ve sklerot oluşumu gözlenirse etmen uyumlu, eğer çarpışma noktasında bariyer oluşursa uyumsuz olarak tanımlanmıştır. Eşleştirme sonucunda 3 MUG saptanmıştır. Misel gelişimi, sklerot sayısı ile sklerot büyüklüğü açısından izolatlar arasında istatistiki olarak farklıklar olduğu saptanmıştır. Yapılan korelasyon analizi sonucunda sklerot sayısı - misel büyümesi ve sklerot sayısı - sklerot çapı arasında negatif; misel büyümesi ile sklerot çapı arasında pozitif bir ilişki bulunmuştur. Tüm izolatların fasulyede yüksek oranda virulent olduğu saptanmıştır. Bu çalışmada S. rolfsii izolatları arasında fenotipik farklılıkların olduğu saptanmıştır.

References

  • Aycock, R. 1966. Stem Rot and Other Diseases Caused by Sclerotium rolfsii or The Status of Rolf’s Fungus After 70 Years. North Carolina State University Agricultural Experiment Station. Tech. Bull. 174.
  • Biçici, M., Çınar, Ö. And Erkiliç, A. 1994. The control of stem rot caused by Sclerotium rolfsii Sacc on peanut by cultural, chemical, physical and biological methods. Tr J. Agric. For. 18: 423-435.
  • Erkılıç A., Güven, B. and Akgün, S.D. 2006. Effects of Some Plant Activators and Plant Materials on Stem Rot Disease of Peanut and Pepper Caused by Sclerotium rolfsii. The journal of Turkish Phytopathol, 34; 15-28.
  • Franke M., Brenneman, T., Stevenson, K. and Padgett. G. 1998. Sensitivity of İsolates of Sclerotium rolfsii from Peanut in Georgia to Selected Fungicides. Plant Dis. 82: 578-583.
  • Harlton, C.E., Levesque, C.A. and Punja, Z.K. 1995. Genetic Diversity in Sclerotium (Athelia) rolfsii and Related Species. Phytopathology. 85: 1269-1281.
  • Kohn, L.M., Stasoviski, E,. Carbone, I., Royers, J. and Anderson, J.B. 1991. Mycelial Incompatibility and Molecular Markers Identify Genetic Variability in Field Populations of Sclerotinia sclerotiorum. Phytopathology. 81: 480-485.
  • Kokub D., Azam, F., Hassan, A., Ansar, M., Asad, M.J. and Khanum, A. 2007. Comparative Growth. Morphological and Molecular Characterization of Indigenous Sclerotium rolfsii Strains Isolated from Different Locations of Pakistan. Pak. J. Bot.. 39(5): 1849-1866.
  • Kown, J.H. and Park, C.S. 2002. Stem Rot of Tomato Caused by Sclerotium rolfsii in Korea. Mycobiology. 30(4): 244-246.
  • Leslie, J.F. 1993. Fungal Vegetative Compatibility. Annu Rev Phytopathol. 31: 127-150.
  • Punja, Z.K. and Grogan, R. G. 1983. Hyphal Interactions and Antagonism among Field Isolates and Single-Basidiospore Strains of Athelia (Sclerotium) rolfsii. Phytopathology. 73: 1279-1284.
  • Punja, Z.K. 1985. The Biology. Ecology. and Control of Sclerotium rolfsii. Annu Rev of Phytopathol. 23: 97-127.
  • Punja, Z. K. and J. E. Rahe. 1992. Sclerotium. In: Singleton. LL. Mihail JD. Rush CM. eds. Methods for research on soilborne phytopathogenic fungi. St. Paul: APS Press. 166–170 pp.
  • Punja, Z.K. and Damiani, A. 1996. Comparative Growth. Morphology. and Physiology of three Sclerotium species. Mycologia. 88: 694-706.
  • Remesal, E. R., Jordán-Ramírez, R.M., Jiménez-Díaz, J. and NavasCortés, A. 2012. Mycelial Compatibility Groups and Pathogenic Diversity in Sclerotium rolfsii Populations From Sugar Beet Crops in Mediterranean-Type Climate Regions. Plant Pathol. 61: 739-753.
  • Saupe, S.J., 2000. Molecular Genetics of Heterokaryon Incompatibility in Filamentous Ascomycetes. Microbiol Mol Biol Rev. 64: 489-502.
  • Xie, C., Huang, C.H., and Vallad, G. E. 2014. Mycelial compatibility and pathogenic diversity among Sclerotium rolfsii isolates in the southern United States. Plant Dis. 98:1685-1694.
  • Yaqub, F. and Shahzad, S. 2005. Pathogencity of Sclerotium rolfsii on Different Crops and Effect of inoculum Density on Colonization Of Mungbean and Sunflower Roots. Pak. J. Bot.. 37(1): 175-180.
There are 17 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

İpek Yaşar 0000-0002-1447-6232

Figen Mert 0000-0002-1603-0226

Publication Date February 15, 2020
Published in Issue Year 2019 Volume: 48 Issue: 1

Cite

APA Yaşar, İ., & Mert, F. (2020). Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters. The Journal of Turkish Phytopathology, 48(1), 1-7.
AMA Yaşar İ, Mert F. Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters. The Journal of Turkish Phytopathology. February 2020;48(1):1-7.
Chicago Yaşar, İpek, and Figen Mert. “Mycelial Compatible Groups of the Sclerotium Rolfsii Isolates and Comparison of Morphological and Pathogenic Characters”. The Journal of Turkish Phytopathology 48, no. 1 (February 2020): 1-7.
EndNote Yaşar İ, Mert F (February 1, 2020) Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters. The Journal of Turkish Phytopathology 48 1 1–7.
IEEE İ. Yaşar and F. Mert, “Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters”, The Journal of Turkish Phytopathology, vol. 48, no. 1, pp. 1–7, 2020.
ISNAD Yaşar, İpek - Mert, Figen. “Mycelial Compatible Groups of the Sclerotium Rolfsii Isolates and Comparison of Morphological and Pathogenic Characters”. The Journal of Turkish Phytopathology 48/1 (February 2020), 1-7.
JAMA Yaşar İ, Mert F. Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters. The Journal of Turkish Phytopathology. 2020;48:1–7.
MLA Yaşar, İpek and Figen Mert. “Mycelial Compatible Groups of the Sclerotium Rolfsii Isolates and Comparison of Morphological and Pathogenic Characters”. The Journal of Turkish Phytopathology, vol. 48, no. 1, 2020, pp. 1-7.
Vancouver Yaşar İ, Mert F. Mycelial Compatible Groups of the Sclerotium rolfsii Isolates and Comparison of Morphological and Pathogenic Characters. The Journal of Turkish Phytopathology. 2020;48(1):1-7.
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