Research Article
BibTex RIS Cite

Syringa vulgaris’in fitokimyasal analizi: LC-ESI-MS/MS ile biyoaktif bileşiklerin kantitatif analizi

Year 2023, Volume: 6 Issue: 2, 75 - 78, 31.12.2023
https://doi.org/10.38059/biodiversity.1312872

Abstract

Tıbbi bitkiler, içeriklerindeki biyoaktif bileşik nedeniyle, gıda ve ilaç sanayinde kullanılmalarını sağlayan önemli biyolojik aktivite gösterirler. Syringa vulgaris çiçeklerinin biyoaktif bileşiklerinin kantitatif analizi, LC-ESI-MS/MS ile belirlendi. Hesperidin (0,33 mg/g ekstrakt) ana ürün olarak belirlendi. Ayrıca S. vulgaris çiçeklerinin metanol ekstraktında naringenin (0,197), klorojenik asit (0,101), o-kumarik asit (0,056), salisilik asit (0,045) ve sinapik asit (0,04) yüksek konsantrasyon olarak belirlenmiştir. Tıbbi bitkiler, içerdikleri biyoaktif bileşikler sayesinde gıda ve ilaç endüstrisinde kullanılmalarını sağlayan önemli biyolojik aktiviteye sahiptirler. S. vulgaris çiçeklerinin biyoaktif bileşik içeriğinin kantitatif analizi LC-ESI-MS/MS yöntemiyle gerçekleştirildi. Bu analiz sonucunda, hesperidin (0,33 mg/g ekstrakt) ana bileşen olarak belirlendi. Ayrıca, S. vulgaris çiçeklerinin metanol ekstraktında naringenin (0,197 3 mg/g ekstrakt), klorojenik asit (0,101 mg/g ekstrakt), o-kumarik asit (0,056 mg/g ekstrakt), salisilik asit (0,045 mg/g ekstrakt) ve sinapik asit (0,04 mg/g ekstrakt) yüksek konsantrasyonlarda tespit edildi. Bu bulgular, S. vulgaris çiçeklerinde bu biyoaktif bileşiklerin bulunduğunu ve gıda ve ilaç sektörlerinde potansiyel uygulamalarının olabileceğini göstermektedir.

References

  • Cavia‐Saiz M, Busto MD, Pilar‐Izquierdo MC, Ortega N, Perez‐Mateos M, Muniz P (2010). Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: A comparative study. Journal of the Science of Food and Agriculture 90:1238-1244.
  • Cragg GM, Newman DJ, Snader KM (1997). Natural products in drug discovery and development. Journal of Natural Products 60:52-60.
  • Demirtas I, Erenler R, Elmastas M, Goktasoglu A (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry 136:34-40.
  • Ellis B (1983). Production of hydroxyphenylethanol glycosides in suspension cultures of Syringa vulgaris. Phytochemistry 22:1941-1943.
  • Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, Demirtas I (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research 30:299-304.
  • Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul‐Enein HY (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters 37:1859-1869.
  • Elmastaş M, Telci İ, Akşit H, Erenler R (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry 40:456-462.
  • Erenler R, Pabuccu K, Yaglioglu AS, Demirtas I, Gul F (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift fur Naturforschung - Section C Journal of Biosciences 71:87-92.
  • Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture 96:822-836.
  • Erenler R, Sen O, Yaglioglu AS, Demirtas I (2016). Bioactivity-guided isolation of antiproliferative sesquiterpene lactones from Centaurea solstitialis l. ssp. solstitialis. Combinatorial Chemistry & High Throughput Screening 19:66-72.
  • Erenler R, Sen O, Yildiz I, Aydın A (2016). Antiproliferative activities of chemical constituents isolated from Thymus praecox subsp. grossheimii (Ronniger) Jalas. Records of Natural Products 10:766-770.
  • Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayir O (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry 39:622-630.
  • Erenler R, Yaman C, Demirtas I, Alma MH (2023). Phytochemical Investigation of Hypericum heterophyllum Flowers: LC-ESI-MS/MS Analysis, Total Phenolic and Flavonoid Contents, Antioxidant Activity. The Natural Products Journal 13:e120123212672.
  • Garg A, Garg S, Zaneveld L, Singla A (2001). Chemistry and pharmacology of the citrus bioflavonoid hesperidin. Phytotherapy Research 15:655-669.
  • Karan T, Dastan T, Baral I, Altuner Z (2016). Effects of Differential Time Applications on Some Cyanobacterial Norharman Production Rates. Cumhuriyet University Faculty of Science 37:398-404.
  • Karan T, Yildiz I, Aydin A, Erenler R (2018). Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products 12:273-283.
  • Lopez-Martinez LX, Oliart-Ros RM, Valerio-Alfaro G, Lee C-H, Parkin KL, Garcia HS (2009). Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. LWT-Food Science and Technology 42:1187-1192.
  • Newman DJ, Cragg GM (2007). Natural products as sources of new drugs over the last 25 years. Journal of Natural Products 70:461-477.
  • Panche AN, Diwan AD, Chandra SR (2016). Flavonoids: an overview. Journal of nutritional science 5:e47. Pezzuto JM (1997). Plant-derived anticancer agents. Biochemical pharmacology 53:121-133.
  • Sahin Yaglioglu A, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S (2013). Antiproliferative activity of pentadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1,8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. roots. Medicinal Chemistry Research 22:2946-2953.
  • Topçu G, Erenler R, Çakmak O, Johansson CB, Çelik C, Chai H-B, Pezzuto JM (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry 50:1195-1199.
  • Varga E, Barabás C, Tóth A, Boldizsár I, Noszál B, Tóth G (2019). Phenolic composition, antioxidant and antinociceptive activities of Syringa vulgaris L. bark and leaf extracts. Natural Product Research 33:1664-1669.
  • Woźniak M, Michalak B, Wyszomierska J, Dudek MK, Kiss AK (2018). Effects of phytochemically characterized extracts from Syringa vulgaris and isolated secoiridoids on mediators of inflammation in a human neutrophil model. Frontiers in Pharmacology 9:349.

Phytochemical analysis of Syringa vulgaris: Quantitative analysis of natural compounds by LC-ESI-MS/MS

Year 2023, Volume: 6 Issue: 2, 75 - 78, 31.12.2023
https://doi.org/10.38059/biodiversity.1312872

Abstract

The significant biological activity exhibited by medicinal plants is attributed to the presence of bioactive compounds, which makes them suitable for applications in the food and pharmaceutical industries. In the case of Syringa vulgaris flowers, a quantitative analysis of its bioactive compound content was conducted using LC-ESI-MS/MS. Among the identified compounds, hesperidin was found to be the majör component, with a concentration of 0.33 mg/g extract. Additionally, the methanol extract of S. vulgaris flowers contained relatively high concentrations of naringenin (0.197 mg/g extract), chlorogenic acid (0.101 mg/g extract), o-coumaric acid (0.056 mg/g extract), salicylic acid (0.045 mg/g extract), and sinapic acid (0.04 mg/g extract). These findings highlight the presence of these bioactive compounds in S. vulgaris flowers and their potential applications in the food and pharmaceutical sectors.

References

  • Cavia‐Saiz M, Busto MD, Pilar‐Izquierdo MC, Ortega N, Perez‐Mateos M, Muniz P (2010). Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: A comparative study. Journal of the Science of Food and Agriculture 90:1238-1244.
  • Cragg GM, Newman DJ, Snader KM (1997). Natural products in drug discovery and development. Journal of Natural Products 60:52-60.
  • Demirtas I, Erenler R, Elmastas M, Goktasoglu A (2013). Studies on the antioxidant potential of flavones of Allium vineale isolated from its water-soluble fraction. Food Chemistry 136:34-40.
  • Ellis B (1983). Production of hydroxyphenylethanol glycosides in suspension cultures of Syringa vulgaris. Phytochemistry 22:1941-1943.
  • Elmastas M, Erenler R, Isnac B, Aksit H, Sen O, Genc N, Demirtas I (2016). Isolation and identification of a new neo-clerodane diterpenoid from Teucrium chamaedrys L. Natural Product Research 30:299-304.
  • Elmastas M, Ozturk L, Gokce I, Erenler R, Aboul‐Enein HY (2004). Determination of antioxidant activity of marshmallow flower (Althaea officinalis L.). Analytical Letters 37:1859-1869.
  • Elmastaş M, Telci İ, Akşit H, Erenler R (2015). Comparison of total phenolic contents and antioxidant capacities in mint genotypes used as spices/Baharat olarak kullanılan nane genotiplerinin toplam fenolik içerikleri ve antioksidan kapasitelerinin karşılaştırılması. Turkish Journal of Biochemistry 40:456-462.
  • Erenler R, Pabuccu K, Yaglioglu AS, Demirtas I, Gul F (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift fur Naturforschung - Section C Journal of Biosciences 71:87-92.
  • Erenler R, Sen O, Aksit H, Demirtas I, Yaglioglu AS, Elmastas M, Telci İ (2016). Isolation and identification of chemical constituents from Origanum majorana and investigation of antiproliferative and antioxidant activities. Journal of the Science of Food and Agriculture 96:822-836.
  • Erenler R, Sen O, Yaglioglu AS, Demirtas I (2016). Bioactivity-guided isolation of antiproliferative sesquiterpene lactones from Centaurea solstitialis l. ssp. solstitialis. Combinatorial Chemistry & High Throughput Screening 19:66-72.
  • Erenler R, Sen O, Yildiz I, Aydın A (2016). Antiproliferative activities of chemical constituents isolated from Thymus praecox subsp. grossheimii (Ronniger) Jalas. Records of Natural Products 10:766-770.
  • Erenler R, Telci I, Ulutas M, Demirtas I, Gul F, Elmastas M, Kayir O (2015). Chemical Constituents, Quantitative Analysis and Antioxidant Activities of Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. Journal of Food Biochemistry 39:622-630.
  • Erenler R, Yaman C, Demirtas I, Alma MH (2023). Phytochemical Investigation of Hypericum heterophyllum Flowers: LC-ESI-MS/MS Analysis, Total Phenolic and Flavonoid Contents, Antioxidant Activity. The Natural Products Journal 13:e120123212672.
  • Garg A, Garg S, Zaneveld L, Singla A (2001). Chemistry and pharmacology of the citrus bioflavonoid hesperidin. Phytotherapy Research 15:655-669.
  • Karan T, Dastan T, Baral I, Altuner Z (2016). Effects of Differential Time Applications on Some Cyanobacterial Norharman Production Rates. Cumhuriyet University Faculty of Science 37:398-404.
  • Karan T, Yildiz I, Aydin A, Erenler R (2018). Inhibition of various cancer cells proliferation of bornyl acetate and essential oil from Inula graveolens (Linnaeus) Desf. Records of Natural Products 12:273-283.
  • Lopez-Martinez LX, Oliart-Ros RM, Valerio-Alfaro G, Lee C-H, Parkin KL, Garcia HS (2009). Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. LWT-Food Science and Technology 42:1187-1192.
  • Newman DJ, Cragg GM (2007). Natural products as sources of new drugs over the last 25 years. Journal of Natural Products 70:461-477.
  • Panche AN, Diwan AD, Chandra SR (2016). Flavonoids: an overview. Journal of nutritional science 5:e47. Pezzuto JM (1997). Plant-derived anticancer agents. Biochemical pharmacology 53:121-133.
  • Sahin Yaglioglu A, Akdulum B, Erenler R, Demirtas I, Telci I, Tekin S (2013). Antiproliferative activity of pentadeca-(8E, 13Z) dien-11-yn-2-one and (E)-1,8-pentadecadiene from Echinacea pallida (Nutt.) Nutt. roots. Medicinal Chemistry Research 22:2946-2953.
  • Topçu G, Erenler R, Çakmak O, Johansson CB, Çelik C, Chai H-B, Pezzuto JM (1999). Diterpenes from the berries of Juniperus excelsa. Phytochemistry 50:1195-1199.
  • Varga E, Barabás C, Tóth A, Boldizsár I, Noszál B, Tóth G (2019). Phenolic composition, antioxidant and antinociceptive activities of Syringa vulgaris L. bark and leaf extracts. Natural Product Research 33:1664-1669.
  • Woźniak M, Michalak B, Wyszomierska J, Dudek MK, Kiss AK (2018). Effects of phytochemically characterized extracts from Syringa vulgaris and isolated secoiridoids on mediators of inflammation in a human neutrophil model. Frontiers in Pharmacology 9:349.

Details

Primary Language English
Subjects Natural Products and Bioactive Compounds
Journal Section Research Articles
Authors

Ramazan ERENLER 0000-0002-0505-3190

Tünay KARAN 0000-0002-9114-8400

İbrahim HOSAFLIOĞLU 0000-0002-0455-0515

Early Pub Date November 30, 2023
Publication Date December 31, 2023
Submission Date June 11, 2023
Acceptance Date August 3, 2023
Published in Issue Year 2023Volume: 6 Issue: 2

Cite

APA ERENLER, R., KARAN, T., & HOSAFLIOĞLU, İ. (2023). Phytochemical analysis of Syringa vulgaris: Quantitative analysis of natural compounds by LC-ESI-MS/MS. Turkish Journal of Biodiversity, 6(2), 75-78. https://doi.org/10.38059/biodiversity.1312872


Creative Commons License
Turkish Journal of Biodiversity is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.