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Characterization of the Essential Oil and Anticandidal Evaluation of Thymus pallasicus Hayek & Velen. from Turkey

Year 2019, Volume: 6 Issue: 4, 1 - 5, 26.12.2019

Abstract



Thymus L. of the Lamiaceae with 220
ssp. is concentrated in the Mediterranean region. Thymus species are well known as “Kekik” in Turkish and are used as
herbal tea and spices, according to recent records, the genus is represented with
40 ssp. where 17 species are endemic to Turkey. 
The study material Thymus pallasicus is one of the endemic species
for Turkey, where the essential oil was obtained from the air dried aerial parts
by hydrodistillation subsequently analyzed by GC-FID and GC-MS, respectively. The
main component was identified as thymol (55.5 %) along with the monoterpenes
p-cymene (19.9 %), γ-terpinene (7.2%), carvacrol (5 %), and 1,8-cineole (%1.4).
The essential oil was also evaluated for its anticandidal activity against five
different standard human pathogenic strains, according to a modified in vitro
CSLI micro dilution method. The minimum inhibitory activity of the essential
oil was 250-500 microgram/mL against the tested Candida sp. suggesting rather weak inhibitory activity when
compared to the standard antifungal fluconazole (0.5-16 microgram/mL). As Thymus species are known for their
antimicrobial activities it is suggested that other clinical and pathogenic
strains should be further evaluated.

References

  • Baytop, T. (1999). Therapy with Medicinal Plants in Turkey, past and Present, 2nd ed. pp. 253-255, Nobel Tıp Kitabevleri, İstanbul.
  • Clinical and Laboratory Standards Institute M27-A2 (2002). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard-Second Edition, CLSI document 22(15).
  • Duman, H. (2000). In: Flora of Turkey and the East Aegean Islands. Vol. 11, Edits., A. Güner, N. Özhatay, T. Ekim and K.H.C. Baser, pp. 198-199, Edinburgh University Press, Edinburgh.
  • Fujita T, Sezik E, Tabata M, Yeşilada E, Honda G, Takeda Y, Tanaka T, Takaishi Y. (1995). Traditional medicine in Turkey VII. Folk medicine and west Black Sea regions. Econ Bot; 49: 406- 422.
  • Güner A., Aslan S., Ekim T., Vural M. and Babaç M.T. (2012). Thymus: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Edits., pp. 599-602 Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
  • Heywood V.H., Brummit R.K., Culham A., Seberg O. (2007). Flowering Plant Families of the Word, p.179-181, Firefly Books: Canada.
  • Hochmuth D. H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • Jales J. (1982). Thymus L. In: Flora of Turkey and the East Aegean Islands. Vol. 7, Edit., P.H. Davis, pp. 349-381, Edinburgh University Press, Edinburgh.
  • McLafferty F. W., Stauffer D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J. Wiley and Sons: New York.
  • Özgen U. (2011). Thymus vulgaris,T. serpyllum,T. zygis. Tedavide Kullanılan Bitkiler FFD Monografları” Nobel Tıp Kitabevleri, s: 667-678, 2. Baskı, Ankara.
  • The Plant List (2013). Version 1.1. Published on the Internet; http://www.theplantlist.org/ (accessed 1st January).
  • Vardar G., Candan F., Sökmen A., Daferea D., Polissious M., Sökmen M., Dönmez E., Tepe B.(2003). Antimicrobial and Antioxidant Activity of the Essential Oil and Methanol Extracts of Thymus pectinatus Fisch. et Mey. var. pectinatus (Lamiaceae), J. of Agricultural and Food Chemistry 51, 63-67.
  • Who Monographs on selected Medicinal Plants (1999). Vol 1, p.295, Genève.
Year 2019, Volume: 6 Issue: 4, 1 - 5, 26.12.2019

Abstract

References

  • Baytop, T. (1999). Therapy with Medicinal Plants in Turkey, past and Present, 2nd ed. pp. 253-255, Nobel Tıp Kitabevleri, İstanbul.
  • Clinical and Laboratory Standards Institute M27-A2 (2002). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard-Second Edition, CLSI document 22(15).
  • Duman, H. (2000). In: Flora of Turkey and the East Aegean Islands. Vol. 11, Edits., A. Güner, N. Özhatay, T. Ekim and K.H.C. Baser, pp. 198-199, Edinburgh University Press, Edinburgh.
  • Fujita T, Sezik E, Tabata M, Yeşilada E, Honda G, Takeda Y, Tanaka T, Takaishi Y. (1995). Traditional medicine in Turkey VII. Folk medicine and west Black Sea regions. Econ Bot; 49: 406- 422.
  • Güner A., Aslan S., Ekim T., Vural M. and Babaç M.T. (2012). Thymus: Türkiye Bitkileri Listesi (Damarlı Bitkiler). Edits., pp. 599-602 Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
  • Heywood V.H., Brummit R.K., Culham A., Seberg O. (2007). Flowering Plant Families of the Word, p.179-181, Firefly Books: Canada.
  • Hochmuth D. H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • Jales J. (1982). Thymus L. In: Flora of Turkey and the East Aegean Islands. Vol. 7, Edit., P.H. Davis, pp. 349-381, Edinburgh University Press, Edinburgh.
  • McLafferty F. W., Stauffer D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, J. Wiley and Sons: New York.
  • Özgen U. (2011). Thymus vulgaris,T. serpyllum,T. zygis. Tedavide Kullanılan Bitkiler FFD Monografları” Nobel Tıp Kitabevleri, s: 667-678, 2. Baskı, Ankara.
  • The Plant List (2013). Version 1.1. Published on the Internet; http://www.theplantlist.org/ (accessed 1st January).
  • Vardar G., Candan F., Sökmen A., Daferea D., Polissious M., Sökmen M., Dönmez E., Tepe B.(2003). Antimicrobial and Antioxidant Activity of the Essential Oil and Methanol Extracts of Thymus pectinatus Fisch. et Mey. var. pectinatus (Lamiaceae), J. of Agricultural and Food Chemistry 51, 63-67.
  • Who Monographs on selected Medicinal Plants (1999). Vol 1, p.295, Genève.
There are 13 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Gülderen Yılmaz 0000-0002-6569-4766

Betül Demirci

Zeki Aytaç

Fatih Demirci

Publication Date December 26, 2019
Published in Issue Year 2019 Volume: 6 Issue: 4

Cite

APA Yılmaz, G., Demirci, B., Aytaç, Z., Demirci, F. (2019). Characterization of the Essential Oil and Anticandidal Evaluation of Thymus pallasicus Hayek & Velen. from Turkey. Natural Volatiles and Essential Oils, 6(4), 1-5.